Journal: ESC Heart Failure

Article Title: MicroRNA‐494 regulates high glucose‐induced cardiomyocyte apoptosis and autophagy by PI3K/AKT/ mTOR signalling pathway

doi: 10.1002/ehf2.14311

Figure Lengend Snippet: MicroRNA (miR)‐494 regulates cell apoptosis and autophagy via the PI3K/AKT/mTOR signalling pathway. The expression level of PI3K/AKT/mTOR pathway‐related proteins in different groups was detected by western blotting. Data are presented as the mean ± SD, n = 3, with three replicates. Replicates = 3. ** P < 0.01 and *** P < 0.001. HG, high glucose; NC, negative control.

Article Snippet: After blocking with 5% skim milk at room temperature for 2 h, the membrane was incubated with corresponding primary antibodies at 4°C: anti‐Bax rabbit monoclonal antibody (1:2000, Abcam, ab32503), anti‐Bcl‐2 rabbit polyclonal antibody (1:2000, Abcam, ab196495), anti‐LC3B rabbit monoclonal antibody (1:1000, Abcam, ab192890), anti‐p62 rabbit monoclonal antibody (1:15 000, Abcam, ab109012), anti‐Beclin‐1 rabbit monoclonal antibody (1:1600, Abcam, ab137020), anti‐p‐PI3K rabbit monoclonal antibody (1:1000, Abcam, ab40776), anti‐p‐AKT rabbit monoclonal antibody (1:4500, Abcam, ab81283), anti‐p‐mTOR rabbit monoclonal antibody (1:2500, Abcam, ab137133), anti‐PI3K mouse monoclonal antibody (1:1800, Abcam, ab140307), anti‐AKT rabbit monoclonal antibody (1:9000, Abcam, ab179463), anti‐mTOR rabbit monoclonal antibody (1:3000, Abcam, ab2732), and anti‐GAPDH rabbit monoclonal antibody (1:8000, Abcam, ab181602).

Techniques: Expressing, Western Blot, Negative Control

Journal: ESC Heart Failure

Article Title: MicroRNA‐494 regulates high glucose‐induced cardiomyocyte apoptosis and autophagy by PI3K/AKT/ mTOR signalling pathway

doi: 10.1002/ehf2.14311

Figure Lengend Snippet: Schematic representation of microRNA (miR)‐494‐dependent regulation of the PI3K/AKT/mTOR pathway involved in the progression of diabetic cardiomyopathy. This diagram shows that during the process of diabetic cardiomyopathy, miR‐494 is up‐regulated and it inhibits the phosphorylation level of the PI3K/AKT/mTOR pathway, which promotes the progression of cardiomyocyte apoptosis and autophagy.

Article Snippet: After blocking with 5% skim milk at room temperature for 2 h, the membrane was incubated with corresponding primary antibodies at 4°C: anti‐Bax rabbit monoclonal antibody (1:2000, Abcam, ab32503), anti‐Bcl‐2 rabbit polyclonal antibody (1:2000, Abcam, ab196495), anti‐LC3B rabbit monoclonal antibody (1:1000, Abcam, ab192890), anti‐p62 rabbit monoclonal antibody (1:15 000, Abcam, ab109012), anti‐Beclin‐1 rabbit monoclonal antibody (1:1600, Abcam, ab137020), anti‐p‐PI3K rabbit monoclonal antibody (1:1000, Abcam, ab40776), anti‐p‐AKT rabbit monoclonal antibody (1:4500, Abcam, ab81283), anti‐p‐mTOR rabbit monoclonal antibody (1:2500, Abcam, ab137133), anti‐PI3K mouse monoclonal antibody (1:1800, Abcam, ab140307), anti‐AKT rabbit monoclonal antibody (1:9000, Abcam, ab179463), anti‐mTOR rabbit monoclonal antibody (1:3000, Abcam, ab2732), and anti‐GAPDH rabbit monoclonal antibody (1:8000, Abcam, ab181602).

Techniques:

Journal: Heliyon

Article Title: AP-1 inhibitor induces ferroptosis via the PI3K/AKT pathway in multiple myeloma cells

doi: 10.1016/j.heliyon.2024.e34397

Figure Lengend Snippet: The PI3K/AKT signaling pathway is involved in AP-1 inhibitor-induced ferroptosis. (A) MM cells were exposed to T-5224 (20 μМ) for 48 h. The protein levels of p -AKT(Ser473), total AKT, p-PI3K (Tyr317), and total PI3K were assessed by western blotting. (B) MM cells were exposed to T-5224 (20 μМ) for 48 h. The protein levels of Bcl2 and Bax in MM cells were assessed by western blotting. (C) MM cells were exposed to T-5224 in the presence or absence of Fer-1 (5 μM) for 48 h, after which the protein levels of p -AKT(Ser473), AKT, p-PI3K (Tyr317), and PI3K were assessed by western blotting. (D) MM cells were exposed to T-5224(20 μM) in the presence or absence of 740 Y–P (20 μM) for 48 h. The protein levels of GPX4 and SLC7A11 in MM cells were assessed by western blotting. β-actin served as the protein loading control. The results are presented as the mean ± standard deviation (SD).

Article Snippet: An anti-β-actin antibody was acquired from Proteintech.Anti-total-PI3K and anti-phospho-PI3KCA (Tyr317) antibodies were acquired from Bioss Co., Ltd. (Beijing, China).

Techniques: Western Blot, Control, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Network Pharmacology and Experimental Validation Reveal the Effects of Chidamide Combined With Aspirin on Acute Myeloid Leukemia-Myelodysplastic Syndrome Cells Through PI3K/AKT Pathway

doi: 10.3389/fcell.2021.685954

Figure Lengend Snippet: CDM combined with ASA inhibited the activation of PI3K/AKT pathway in AML-MDS cells. (A) Western blot analysis of PI3K, p-PI3K AKT, p-AKT. (B) Western blotting of PI3K, p-PI3K, AKT and p-AKT. (C) Western blotting of CDK2, CDK4 and p21. (D) Western blotting of Bcl-2, caspase-3 and cleaved caspase-3. IGF-1 reversed the effect of CDM combined with ASA on AML-MDS cells. β-actin served as a loading control. Data are mean ± SD of three independent experiments. “ ∗ ” indicates a significant difference relative to the control group ( ∗ p < 0.05), “&” indicates a significant difference relative to ASA-treated group (& p < 0.05), “NS” indicates no significant difference relative to the control group.

Article Snippet: PI3K (bsm-33219M), p-PI3K (AB1235888), and Caspase-3 (bs-0081R) from Bioss (Beijing, China).

Techniques: Activation Assay, Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Network Pharmacology and Experimental Validation Reveal the Effects of Chidamide Combined With Aspirin on Acute Myeloid Leukemia-Myelodysplastic Syndrome Cells Through PI3K/AKT Pathway

doi: 10.3389/fcell.2021.685954

Figure Lengend Snippet: A schematic representation of the proposed pathway responsible for CDM combined with ASA in AML-MDS cells. (A) The predictive pathways of CDM and ASA in AML-MDS through network pharmacology. (B) CDM combined with ASA could inhibited the activation of PI3K/AKT signaling pathways, and then affected the expression of cell cycle and apoptosis-related proteins to induce cell cycle arrest and apoptosis in AML-MDS cells through experimental validation.

Article Snippet: PI3K (bsm-33219M), p-PI3K (AB1235888), and Caspase-3 (bs-0081R) from Bioss (Beijing, China).

Techniques: Activation Assay, Expressing

Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Comparison of the quantity of collagen types III (%) in the tendon tissue between the TENS and Simulation groups. Data expressed in mean and standard deviation.

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Comparison, Standard Deviation

Journal: Brazilian Journal of Physical Therapy

Article Title: Can transcutaneous electrical nerve stimulation improve achilles tendon healing in rats?

doi: 10.1590/bjpt-rbf.2014.0107

Figure Lengend Snippet: Measures of variables related to group, assessment, and results of Analysis of Variance (ANOVA).

Article Snippet: The expression of collagen I and III, observed in the immunohistochemical reaction with the polyclonal antibodies for the collagen types I and III (Affinity Purified Anti-Collagen Type I and Type III, Rabbit Anti-Human, Rockland Inc., Gilbertsville, PA, USA), was analyzed by quantifying the area corresponding to each type of collagen.

Techniques: Standard Deviation